Fraktionierung von Peptiden

Verbesserung der Proteinidentifikation durch LC-MS/MS in komplexen biologischen Proben wie Plasma durch Peptidfraktionierung bei basischem pH.

02 sf peptide fractionation v2

BioSPE™ PepFrac wird für die Peptidfraktionierung nach Hydrophobizität bei basischem pH-Wert verwendet. Da diese Fraktionierungsmethode orthogonal zur Peptidtrennung durch Umkehrphasen-Flüssigkeitschromatographie ist, wird die Komplexität der Proben für die Tiefenproteomsequenzierung und quantitative Analyse reduziert.

Der BioSPE™ PepFrac-Kit besteht aus:

  • Fraktionierungssäulen auf der Grundlage eines innovativen Umkehrphasenharzes
  • Adaptern (für die Zentrifugation von Spitzen) und/oder Sammelwerkzeugen (Zentrifugenröhrchen oder Sammelplatten). Jeder Kit enthält neun Sammeltools (eines für die Konditionierungs-/Lade-/Waschschritte und eines für jede Elutionsfraktion)
  • Ein detailliertes Protokoll für eine schnelle und effiziente Peptidfraktionierung (bis zu 8 Fraktionen)
  • Triethylamin (TEA)-Lösung

Um festzustellen, welches Format und welche Kapazität am besten für Ihre Bedürfnisse geeignet sind, sehen Sie sich bitte die Tabellen auf der Registerkarte “Formate und Kapazitäten” an.

PRODUKTLISTE

Produktname Produktpreis Product SKU Warenkorb-Aktionen
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.H8.1
kit 96 plate microelution biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate for microelution & collection plates

96 Wellplate for peptide fractionation at basic pH - Tip-shaped wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-µ96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - Standard Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.S8.1
kit 96 plate 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – 96 wellplate & collection plates

96 Wellplate for peptide fractionation at basic pH - 1mL wells - High Capacity - 1/pk + Collection plate - 1mL - 9/pk + Triethylamine 0.1% (200mL)

Ref. : KT-Coll-96W-PepFrac.H8.1
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.200.96
kit tips 200ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 200µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 200µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.200.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL & spin adapters

Kit of tips for peptide fractionation at basic pH - 10µL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.10.96
kit tips 10ul biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 10µL Single Cell & spin adapters

Kit of tips for peptide fractionation at basic pH in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.SC8.10.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - Standard Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.S8.1000.96
kit tips 1ml biospe pepfrac 600x600

BioSPE™ PepFrac – Tips 1mL & spin adapters

Kit of tips for peptide fractionation at basic pH - 1mL - High Capacity - 96/pk + spin adapters - 96/pk + collection tubes - 864/pk + Triéthylamine 0.1% (100mL)

Ref. : KT-Adapt-Tips-PepFrac.H8.1000.96
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.S8.50
kit spin micro biospe pepfrac 600x600

BioSPE™ PepFrac – µSpin

µSpin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : µSpin-PepFrac.H8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.S8.50
kit spin mini biospe pepfrac 600x600

BioSPE™ PepFrac – Spin

Spin columns for peptide fractionation at basic pH - High Capacity - 50/pk + collection tubes - 450/pk + Triethylamine 0.1% (100mL)

Ref. : Spin-PepFrac.H8.50
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.S8.25
kit spin 15ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 15mL

Spin columns for peptide fractionation at basic pH - High Capacity - 15mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin15-PepFrac.H8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - Standard Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.S8.25
kit spin 50ml biospe pepfrac 600x600

BioSPE™ PepFrac – Spin 50mL

Spin columns for peptide fractionation at basic pH - High Capacity - 50mL - 25/pk + collection tubes - 225/pk + Triethylamine 0.1% (100mL)

Ref. : Spin50-PepFrac.H8.25
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.S8.50
cartridge biospe 1ml 600x600

BioSPE™ PepFrac – Cartridges 1mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 1mL - 50/pk + Triethylamine 0.1% (100mL)

Ref. : CAR1-PepFrac.H8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.S8.50
cartridge biospe 3ml 600x600

BioSPE™ PepFrac – Cartridges 3mL

SPE cartridges for peptide fractionation at basic pH - High Capacity - 3mL - 50/pk + Triethylamine 0.1% (200mL)

Ref. : CAR3-PepFrac.H8.50
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - Standard Capacity - 6mL - 25/pk + Triethylamine 0.1% (200mL)

Ref. : CAR6-PepFrac.S8.25
cartridge biospe 6ml 600x600

BioSPE™ PepFrac – Cartridges 6mL

SPE cartridges for peptide fractionation at basic pH - 6mL - 25/pk

Ref. : CAR6-PepFrac.H8.25