Peptidentsalzung
Reinigung und Entsalzung von Peptiden in Bottom-up-Proteomik-Ansätzen mit BioSPE® PurePep.
BioSPE® PurePep wird für eine Entsalzungswirkung von Peptiden nach enzymatischer Verdauung und vor der LC-MS/MS-Analyse verwendet. Jusqu’à 97% der Proteine ohne Identifizierung nach der Entsalzung von HeLa auf BioSPE® PurePep, mit einer guten Rekuperation der Peptide auf dem ganzen Polargamme und einer guten Reproduktion (RSD < 3%).
Gießen Sie die Dessalage des Peptids weniger plus Hydrophile, wir empfehlen Ihnen die Verwendung von BioSPE® PurePep Broad, um die Wirksamkeit kleiner Peptide zu erhalten. BioSPE® PurePep ist breit stabil in extremen pH-Bedingungen (pH 0-14) und hält den SPE-Prozess ohne Folgen für die Ergebnisse aufrecht.
Die Kits BioSPE® PurePep und BioSPE™ PurePep Broad bestehen aus:
- Entsalzungssäulen
- Adaptern (für die Zentrifugation von Spitzen) und/oder Sammelwerkzeugen (Zentrifugenröhrchen oder Sammelplatten). Jedes Kit enthält zwei Entnahmehilfen (eine für die Konditionierungs-/Lade-/Waschschritte und eine für den Elutionsschritt)
- Ein detailliertes Protokoll für eine schnelle und einfache Entsalzung
Um festzustellen, welches Format und welche Kapazität am besten für Ihre Bedürfnisse geeignet sind, sehen Sie sich bitte die Tabellen auf der Registerkarte „Formate und Kapazitäten“ an.
- Keine Lagerbeschränkung (bei Raumtemperatur trocken lagern)
- Verschiedene Formate zur Anpassung an Probenvolumen und Durchsatz verfügbar (SPE-Tips, Spin-Säulen, 96- & 384-Wellplates und Kartuschen)
- Zwei Kapazitäten für jedes Format verfügbar, abhängig von Ihrer Peptidmenge
Details zu den Lade- und Elutionsvolumina für unsere verschiedenen BioSPE® PurePep-Produkte, um das beste Produkt für Ihr Probenvolumen zu finden.
Informationstabelle zu den Kapazitäten (maximale Menge an Peptiden/Proteinen, die zurückgehalten werden können) unserer verschiedenen BioSPE® PurePep-Produkte :
- Bei Standardkapazität enthalten die Produktbezeichnungen den Buchstaben S (z. B. Tips-PurePep.S.200.96).
- Für hohe Kapazität enthalten die Produktreferenzen den Buchstaben H (z. B. Tips-PurePep.H.200.96)
Literatur
Hijazi, H. et al. (2025) ‚Mind Your Spectra: Points to be Aware of When Validating the Identification of Isobaric Histone Peptidoforms‚, Journal of Proteome Research.
In ihrer jüngsten Veröffentlichung untersuchen Hassan M. Hijazi et al. die Identifizierung der mausspezifischen Histonvarianten H3mm7 und H3mm13 aus Gehirn- und Hodenextrakten mittels LC-MS/MS.
Um eine genaue Erkennung posttranslationaler Modifikationen wie Acetylierung und Lactylierung zu ermöglichen, wurde die Peptidreinigung mit Affinisep BioSPE® PurePep durchgeführt, das sich als äußerst wirksam bei der Entfernung störender Substanzen und der Erhaltung modifizierter Peptide erwies.
Dieser Ansatz verbesserte die Qualität des MS/MS-Spektrums, insbesondere für kritische Ionen wie b1 und CycIm, und half bei der Bewältigung von Herausforderungen im Zusammenhang mit isobaren Varianten, die durch unkontrollierte Modifikationen wie Propionylierung entstehen.
Verwendetes Produkt: BioSPE® PurePep Tips 200µl (KT-Adapt-Tips-PurePep.H.200.96)
Anwendungshinweise
- Proteomik
Estimation of the working range on BioSPE™PurePep SPE Tips – Standard capacity
Neu!
Peptide desalting with BioSPE™ PurePep
Fraktionierung von Peptiden
Verbesserung der Proteinidentifikation durch LC-MS/MS in komplexen biologischen Proben wie Plasma durch Peptidfraktionierung bei basischem pH.
Entfernung von Magnetkügelchen
Entfernung von Magnet-Beads aus Proben nach der Probenvorbereitung mit Einzeltopf und Festphasenverstärkung (SP3).
Aufreinigung von intakten Proteinen
Reinigung intakter Proteine bei Top-down-Proteomik-Ansätzen.
PRODUKTLISTE
| Product Image | Produktname | Product Description | Produktpreis | Warenkorb-Aktionen | |
|---|---|---|---|---|---|
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BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.S.1 |
96 Wellplate for peptide desalting - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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BioSPE™ PurePep – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep.H.1 |
96 Wellplate for peptide desalting - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate & collection plates
Ref. : KT-Coll-96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - 1mL wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.SC.10.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.1000.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.H.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep Broad – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep-Broad.S.200.96 |
Kit of tips for peptide purification on broad pH range (pH 0-14) - 200µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.H.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep.S.1 |
96 Wellplate for peptide desalting - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.H.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - High Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep Broad – 96 wellplate for microelution & collection plates
Ref. : KT-Coll-µ96W-PurePep-Broad.S.1 |
96 Wellplate for peptide purification on broad pH range (pH 0-14) - Tip-shaped wells - Standard Capacity - 1/pk + Collection plates - 1mL - 2/pk |
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|
BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.10.96 |
Kit of tips for peptide desalting - 10µL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 10µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.10.96 |
Kit of tips for peptide desalting - 10µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 10µL Single Cell & spin adapters
Ref. : KT-Adapt-Tips-PurePep.SC.10.96 |
Kit of tips for peptide desalting in single-cell proteomics - 10µL - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.1000.96 |
Kit of tips for peptide desalting - 1mL - Standard capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 1mL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.1000.96 |
Kit of tips for peptide desalting - 1mL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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|
BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.S.200.96 |
Kit for peptide desalting containing: SPE Tips 200µL - Standard capacity - 96/pk | Spin adapters - 96/pk | 2mL collection tubes - 192/pk |
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BioSPE™ PurePep – Tips 200µL & spin adapters
Ref. : KT-Adapt-Tips-PurePep.H.200.96 |
Kit of tips for peptide desalting - 200µL - High capacity - 96/pk + spin adapters - 96/pk + collection tubes - 192/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.S.50 |
Spin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Cartridges 3mL
Ref. : CAR3-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep Broad – Cartridges 6mL
Ref. : CAR6-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 6mL - Standard capacity - 50/pk |
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BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.S.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Spin
Ref. : Spin-PurePep-Broad.H.50 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 15mL
Ref. : Spin15-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 15mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.H.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – Spin 50mL
Ref. : Spin50-PurePep-Broad.S.25 |
Spin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50mL - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.S.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep Broad – µSpin
Ref. : µSpin-PurePep-Broad.H.50 |
µSpin columns for peptide purification on broad pH range (pH 0-14) - High capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.S.50 |
µSpin columns for peptide desalting - Standard capacity - 50/pk + collection tubes - 100/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.S.50 |
SPE cartridges for peptide desalting - 1mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 1mL
Ref. : CAR1-PurePep.H.50 |
SPE cartridges for peptide desalting - 1mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.S.50 |
SPE cartridges for peptide desalting - 3mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 3mL
Ref. : CAR3-PurePep.H.50 |
SPE cartridges for peptide desalting - 3mL - High capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.S.50 |
SPE cartridges for peptide desalting - 6mL - Standard capacity - 50/pk |
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BioSPE™ PurePep – Cartridges 6mL
Ref. : CAR6-PurePep.H.50 |
SPE cartridges for peptide desalting - 6mL - High capacity - 50/pk |
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BioSPE™ PurePep – Spin
Ref. : Spin-PurePep.H.50 |
Spin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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|
BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.H.25 |
Spin columns for peptide desalting - 15mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 15mL
Ref. : Spin15-PurePep.S.25 |
Spin columns for peptide desalting - 15mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.H.25 |
Spin columns for peptide desalting - 50mL - High capacity - 25/pk + collection tubes - 50/pk |
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BioSPE™ PurePep – Spin 50mL
Ref. : Spin50-PurePep.S.25 |
Spin columns for peptide desalting - 50mL - Standard capacity - 25/pk + collection tubes - 50/pk |
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|
BioSPE™ PurePep – µSpin
Ref. : µSpin-PurePep.H.50 |
µSpin columns for peptide desalting - High capacity - 50/pk + collection tubes - 100/pk |
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|
BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.H.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - High capacity - 50/pk |
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|
BioSPE™ PurePep Broad – Cartridges 1mL
Ref. : CAR1-PurePep-Broad.S.50 |
SPE cartridges for peptide purification on broad pH range (pH 0-14) - 1mL - Standard capacity - 50/pk |
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